NCAM; RB X CH-50UG

Analysis Note

ControlNeuraminidase-treated mouse brain membrane.

Application

Research Sub CategoryGrowth Cones & Axon Guidance

Detect Neural Cell Adhesion Molecule using this Anti-Neural Cell Adhesion Molecule Antibody validated for use in ELISA, IF, IH, FUNC & WB.

Research CategoryNeuroscience

Immunohistochemistry:
A previous lot was shown to work with a wide range of fixatives. Will detect chicken NCAM using a concentration of 1 µg/mL and mouse NCAM using a concentration of 2 µg/mL.

Immunofluorescence:
A previous lot of this antibody was used in Immunofluorescence.

Function blocking:
A previous lot of this antibody was used in Function blocking.

ELISA:
0.3 µg/mL of a previous lot was used.

Optimal working dilutions must be determined by end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The Immunoglobulin superfamily of cell adhesion molecules is related to the immunoglobulins proteins by structure. The family contains over a 100 members, which include ICAM (intercellular adhesion molecule), NCAM (neural cell adhesion molecule) and NgCAM (neural-glial cell adhesion molecule). Both NCAM and NgCAM are calcium independent adhesion molecules. NCAM, the first CAM to be characterized, is expressed on all neurons from very early in development. It binds homophilically to NCAM on adjacent cells and has a variety of functions during neural development. One of these important functions is to hold developing neurites together during neuron outgrowth and formation of neural connections. The adhesive properties of NCAM are modulated during development and plasticity by the presence of the regulated carbohydrate, polysialic acid (PSA). The post-translational addition of alpha-2,8-linked PSA (NCAM-PSA) appears to contribute to synaptic plasticity during learning and memory formation and to the mechanisms of nerve regeneration.

Immunogen

Highly purified chicken NCAM

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

ImmunoAffinity Purified

Purified rabbit polyclonal in buffer containing PBS containing no preservatives.

Quality

Routinely evaluated by Western Blot on mouse brain lysates.

Western Blot Analysis: 1:1000 dilution of this lot detected NCAM on 10 µg of mouse brain lysate.

Specificity

Expected to cross-react with Chicken, Human, and rat.

Neural Cell Adhesion Molecule (NCAM). MW is 200-250 kDa; when treated with Neuraminidase, 140-180 kDa. A breakdown product of 160 kDa may also be visible.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

MW is 200-250 kDa; when treated with Neuraminidase, 140-180 kDa A breakdown product of 160 kDa may also be visible.